The second edition of the Brazilian Cyclist Profile survey was performed by the NGO Ativo Transporte and LABMOB, counting on an extensive network of collaborating organizations that took to the field more than 140 researchers to the streets between September 2017 and April 2018. In this edition, besides Brazil, cities of Argentina and Colombia were included. In Brazil, 7,644 cyclists were interviewed in 25 cities of different Brazilian regions: Afuá (PA), Antonina (PR), Aracaju (SE), Belém (PA), Brasília (DF), Cáceres , Curitiba (PR), Florianópolis (SC), Gurupi (TO), Ilha Solteira (SP), Mambaí (GO), Manaus (AM), Niteroi (RJ), Palmas (TO), Pedro Leopoldo (MG), Pomerode SC), Porto Alegre (RS), Recife (PE), Rio de Janeiro (RJ), São Fidelis (RJ), São Paulo (SP), Sorocaba (SP), Tamandaré (PE), and Tarauacá (AC). In Argentina, the research was carried out in three cities, being Rosario, Salta and Santa Rosa. In Colombia, four cities joined us: Bucaramanga, Medellin, Popayan and Villavicencio.